Results the optimum process of ultrasonic extraction of total flavonoids from Ephedra sinica was as follows: ultrasonic extraction twice, 10 times the amount of 75% ethanol, ultrasonic time was 30 min, and the content of total flavonoids in Ephedra sinica was 0.255%.
Conclusion the method is simple, accurate and reliable, and can provide reference for the improvement of the quality standard of Zukamu granules### Objective to study the optimum extraction process of total flavonoids from Ephedra Sinica.
Methods the volatile oil was extracted by steam distillation.
Our advantages: direct connection with social leaders, stable employment, ten-year reputation brand 230 ℃, ECD temperature 300 ℃; The ion source is EI source, with dissociation voltage of 70ev, ion source temperature of 240 ℃, transmission line temperature of 250 ℃, four-stage rod temperature of 150 ℃, scan mode is adopted, and the scanning mass charge ratio range is m / Z45 ~ 400.
Conclusion the method is simple, rapid and suitable for the extraction of total flavonoids from Ephedra Sinica### Objective to establish a GC-MS combined with retention index method for the analysis of volatile chemical components in dried fruits of Chaetomium japonicum.
Among them, the chemical components with a content of more than 4.00% are 1,2,3,4-tetrahydro-1,1,6-trimethyl-naphthalene (13.07%), 4-methyl-5-pent-1,3-tetrahydrofuran-2-one (12.67%).
Results 42 kinds of volatile chemical components were identified from the dried fruits, accounting for 95% of the total content.
The relative percentage of volatile components in the dried fruit was determined by gas chromatography-mass spectrometry (GC-MS) combined with retention index method and peak area normalization method.
Methods the extraction process was optimized by orthogonal experiment.
Results 20 organochlorine pesticides were well separated (resolution ≥ 1.512), the average recovery of each component was 72.4% ~ 108.9% (n = 6), and the organochlorine pesticides were 0.002 ~ 0.500 μ There was a good linear relationship in the concentration range of g / ml (R2 ≥ 0.9971).
Publication of medical journals (provincial journals, national journals, core journals of Peking University, SCI journals, etc.), topics, patents, monographs, scientific research projects.
The content of total flavonoids in Ephedra was determined by NaNO2 Al (NO3) 3-NaOH method.
